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Analytical separation and detection methods for flavonoids.

Eva de Rijke1, Pieter Out, Wilfried M A Niessen

  • 1Quest International, Department of Analytical Research and Development, Huizerstraatweg 28, 1411 GP Naarden, The Netherlands. drijke@few.vu.nl

Journal of Chromatography. A
|February 17, 2006
PubMed
Summary

This review covers methods for separating and detecting flavonoids, important plant compounds. It highlights techniques like liquid chromatography and mass spectrometry for analyzing flavonoids in food, drinks, and biological samples.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacognosy

Background:

  • Flavonoids are significant natural compounds with notable biological and physiological effects.
  • Their analysis is crucial across various fields, including food science, agriculture, and medicine.

Purpose of the Study:

  • To provide a comprehensive overview of separation and detection methodologies for flavonoids.
  • To discuss the application of these methods in diverse matrices such as plants, food, beverages, and biological fluids.
  • To emphasize recent advancements, particularly in structural characterization.

Main Methods:

  • Sample preparation techniques relevant to flavonoid extraction.
  • Chromatographic methods including Liquid Chromatography (LC), Gas Chromatography (GC), Capillary Electrophoresis (CE), and Thin-Layer Chromatography (TLC).

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  • Detection techniques such as Diode-Array UV (DAD UV) and tandem Mass Spectrometry (MS/MS).

    • Main Results:

    • Detailed discussion on various separation and detection strategies for flavonoids.
    • Emphasis on the utility of tandem-mass spectrometry (MS/MS) for flavonoid structure elucidation.
    • Exploration of combined detection methods (DAD UV, MS/MS, NMR) for unambiguous identification.

    Conclusions:

    • The review consolidates current knowledge on flavonoid analysis, offering insights into established and emerging techniques.
    • Highlights the importance of advanced analytical methods for comprehensive flavonoid profiling and identification.
    • Underscores the trend towards hyphenated techniques for enhanced structural characterization and identification accuracy.