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Production, isolation and characterization of human profilin from Saccharomyces cerevisiae
P Aspenström1, I Lassing, R Karlsson
1Department of Developmental Biology, Uppsala University, Sweden.
Journal of Muscle Research and Cell Motility
|April 1, 1991
Abstract:
The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.