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Updated: Aug 11, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
Published on: April 18, 2016
Identification, cloning, and expression of potential diagnostic markers for Q fever
1Rickettsial Disease Department, Naval Medical Research Center, Silver Spring, MD 20910, USA. chaoc@nmrc.navy.mil
Abstract:
The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, and downstream application.
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