Related Experiment Video
Updated: Aug 11, 2026

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
High-performance liquid chromatography-electronspray ionization mass spectrometry for determination of tiopronin in
1Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, PR China.
Abstract:
A simple and sensitive HPLC/ESI-MS method for the determination of tiopronin in human plasma was described. Vitamin C and 2-mercaptoethanol (2-Me) were used as the reducer and the stabilitizer to release and stabilify tiopronin from a dimmer and mix forms with endogenous thiols in the treatment of plasma samples. The analytes were separated on a Johnson Spherigel analytical column packed with 5 microm C8 silica, using the formic acid aqueous solution (pH 4.5) including tris(hydroxymethyl) aminomethane (Tris) and 2-Me (0.5 and 1 mM, respectively) as a mobile phase. Cyclamate was used as the internal standard (I.S.) for the quantification of tiopronin. The correlation coefficient of the calibration curve were better than 0.998 in the range of 0.107-5.35 microg/ml in human plasma. The limit of quantification (LOQ) was 0.107 microg/ml (S/N 10:1, RSD 7.1%). The inter-day and intra-day accuracy was below 7.1 and 6.8%, respectively. As a preliminary application, this method has been successfully applied to the determination of tiopronin in the human plasma.
More Related Videos
05:52Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
Published on: January 12, 2024
09:36Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
High-Performance Liquid Chromatography: Types of Detectors
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Ion-Exchange Chromatography