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EPR study of the hydrophobic interaction of spectrin with fatty acids
S Streichman1, E Kahana, B L Silver
1Department of Hematology, Rambam Medical Center, Haifa, Israel.
Biochimica Et Biophysica Acta
|July 1, 1991
Abstract:
The hydrophobic interaction between spin-labelled stearic acid and spectrin was studied by electron paramagnetic resonance (EPR) and fluorescence quenching. The results are quantitatively interpreted in terms of two types of binding site on spectrin. A comparison between the results of the EPR and fluorescence experiments show the drawback of the fluorescence method in binding studies.