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Updated: Aug 11, 2026
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Factor V Kuwait: a novel mutation in the coagulation factor V gene discovered in Kuwait
Mehrez M Jadaon1, Ali A Dashti, Hend L Lewis
1Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University, Kuwait. mehrez@hsc.edu.kw
Objective:
It was the aim of this study to report a new point mutation in the clotting factor V gene in the general Arab population.
Subjects And Methods:
The HR2 haplotype was tested in 288 Arabs living in Kuwait--188 patients with venous thromboembolic disorders (VTE) and 100 healthy subjects--using polymerase chain reaction and restriction fragment length polymorphism techniques. The presence of the new mutation was verified by DNA sequencing.
Results:
Two (1.06%) VTE patients had guanine instead of the wild-type adenine at nucleotide number 3935 (A3935G) of the factor V gene. This mutation caused a histidine to arginine change in amino acid number 1254 of the factor V molecule. The new mutation is termed 'factor V Kuwait' (His1254Arg) and was absent in the 100 healthy subjects.
Conclusion:
It appears that factor V Kuwait could be a risk factor for developing VTE in Arabs. A larger study is needed to confirm this observation.
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