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Updated: Aug 11, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Non-enzymatic regeneration of nicotinamide and flavin cofactors for monooxygenase catalysis
F Hollmann1, K Hofstetter, A Schmid
1Degussa Care & Surface Specialties, Goldschmidt AG, Goldschmidtstrasse 100, 45127 Essen, Germany.
Abstract:
Biocatalytic oxygenation chemistry is a rapidly evolving field in which monooxygenases are the tools of choice. Monooxygenases catalyze many industrially important synthetic transformations; however, their use in preparative applications is hampered by their intrinsic requirement for reducing equivalents. As a result, non-enzymatic strategies--where the reducing equivalents are introduced directly into the catalytic cycle--are being developed to supersede the well-established enzymatic NAD(P)H regeneration systems currently in use. In this review we summarize and evaluate recent achievements in this area.
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