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Updated: Aug 11, 2026

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distribution
Manojkumar A Puthenveedu1, Collin Bachert, Sapna Puri
1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Abstract:
The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is mediated by specific membrane-fusion events that occur during Golgi assembly, and require the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-dependent lateral cisternal-fusion reactions are necessary to achieve uniform distribution of enzymes in the Golgi ribbon. The membrane continuity created by ribbon formation facilitates optimal processing conditions in the biosynthetic pathway.
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