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Updated: Aug 11, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes
Zulfiqar Ali Raza1, Asma Rehman, Muhammad Saleem Khan
1Polymer Chemistry Laboratory, National Centre of Excellence in Physical Chemistry, University of Peshawar, 25210 Peshawar, Pakistan.
Abstract:
Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l(-1)) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l(-1)) and rhamnolipid production (8.50 g l(-1)), at 10 d of incubation with the specific growth rate of 0.316 h(-1) and specific product yield of 0.597 g g(-1) h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m(-1).
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