Related Experiment Video
Updated: Oct 3, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
Published on: August 25, 2020
Immunohistochemistry
1Hedley Atkins/Cancer Research UK Breast Pathology Laboratory, Guy's Hospital, London.
Abstract:
Immunohistochemistry allows specific proteins to be visualized while retaining cellular or tissue structure. Sensitivity of the technique has improved over the years from a single layer of labeled antibody to amplified, labeled polymer-based systems, which enable low levels of antigen to be detected in different types of tissue preparation. However, alongside selecting the most appropriate methods of antigen recovery and enhanced signal amplification is the need to show specificity. As described in this chapter, pertinent and reliable control material must be used to both optimize and monitor performance of novel breast-associated antibodies.
More Related Videos
09:11Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
Published on: November 14, 2016
08:40Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Related Concept Videos
Immunocytochemistry and Immunohistochemistry
These...
Immunogold Electron Microscopy
Immunofluorescence Microscopy