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Detection of HER2 gene amplification by fluorescence in situ hybridization in breast cancer

John M S Bartlett1, Amanda Forsyth

  • 1Division of Cancer Sciences and Molecular Pathology, University Department of Surgery, Endocrine Cancer Group and HER2 Reference Laboratory, University of Glasgow, Glasgow Royal Infirmary, UK.

Methods in Molecular Medicine
|February 24, 2006
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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