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Updated: Aug 11, 2026
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
Published on: August 15, 2013
Nine fluorescence parameter analysis on a four-color fluorescence activated flow cytometer
Ursula Frischmann1, Werner Müller
1German Research Centre for Biotechnology, Department of Experimental Immunology, Mascheroder Weg 1, D-38124 Braunschweig. urd@gbf.de
Background:
In many cases a frequent monitoring of blood is important, e.g. during an infection. Often the availability of blood is the limiting factor.
Methods:
50 microl blood were stained and analyzed using a standard four-color cytometer. Percentages of leukocytes were calculated by FlowJo software.
Results:
Our protocol allows the differentiation of B cells, T cells, NK cells, neutrophils, and monocytes/macrophages in small volumes of blood.
Conclusions:
Using nine fluorochrome-labeled antibodies and a specific gating strategy we were able to differentiate the main immune cells in minute amounts using one staining step.
