Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging
Ellen M Quardokus1, Diane C Saunders2,3, Elizabeth McDonough4
1Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.
Nature Methods
|July 19, 2023
Summary
Multiplexed antibody imaging offers detailed tissue analysis. Community-validated organ mapping antibody panels reduce costs and accelerate the creation of a Human Reference Atlas.
Area of Science:
- Biotechnology
- Molecular Biology
- Immunohistochemistry
Background:
- Multiplexed antibody-based imaging is crucial for characterizing molecular and cellular organization within tissues.
- Recent advancements permit high-parameter data acquisition, analyzing over 60 targets simultaneously.
Purpose of the Study:
- To introduce organ mapping antibody panels as community-validated resources.
- To address the high expertise and capital costs associated with constructing antibody panels for multiplexed imaging.
Main Methods:
- Development and validation of organ mapping antibody panels.
- Application of these panels in high-parameter multiplexed imaging studies.
Main Results:
- Organ mapping antibody panels are established as valuable community resources.
- These panels significantly reduce the time and financial investment required for panel construction.
Conclusions:
- Community-validated antibody panels enhance reproducibility and accelerate scientific discovery.
- These resources are instrumental in building a comprehensive Human Reference Atlas.
