Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemoglobin adducts in workers exposed to benzidine and azo dyes.

Armin Beyerbach1, Nathaniel Rothman, Vijai K Bhatnagar

  • 1Institute of Environmental and Occupational Toxicology, Casella Postale 108, CH-6780 Airolo, Switzerland.

Carcinogenesis
|February 25, 2006
PubMed
Summary

Benzidine and azo dyes exposure in Indian workers led to hemoglobin adducts of benzidine, 4-aminobiphenyl, and aniline. Higher adduct levels were found in benzidine production workers, indicating significant occupational exposure risks.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quo vadis human biomonitoring?

Chemico-biological interactions·2025
Same author

NIBR-LTSi is a selective LATS kinase inhibitor activating YAP signaling and expanding tissue stem cells in vitro and in vivo.

Cell stem cell·2024
Same author

New Method to Biomonitor Workers Exposed to 1,6-Hexamethylene Diisocyanate.

Chemical research in toxicology·2022
Same author

Literature review and evaluation of biomarkers, matrices and analytical methods for chemicals selected in the research program Human Biomonitoring for the European Union (HBM4EU).

Environment international·2022
Same author

Quo vadis blood protein adductomics?

Archives of toxicology·2021
Same author

BET bromodomain inhibitors regulate keratinocyte plasticity.

Nature chemical biology·2021

Area of Science:

  • Occupational Health
  • Toxicology
  • Chemical Carcinogenesis

Background:

  • Benzidine (Bz) is a known human carcinogen.
  • Azo dyes can be synthesized using Bz and may release it metabolically.
  • Occupational exposure to Bz and its derivatives poses significant health risks.

Purpose of the Study:

  • To investigate hemoglobin (Hb) adducts in Indian workers producing Bz and azo dyes.
  • To quantify specific arylamine adducts resulting from occupational exposure.
  • To correlate adduct levels with exposure sources and potential health implications.

Main Methods:

  • Gas chromatography-mass spectrometry (GC-MS) was used for identification and quantification of Hb adducts.
  • Analysis included benzidine (Bz), N-acetylbenzidine (AcBz), 4-aminobiphenyl (4ABP), and aniline.

Related Experiment Videos

  • Study involved 33 exposed workers, categorized into Bz workers (n=15) and dye workers (n=18).

    • Main Results:

    • Hb adducts of Bz, AcBz, 4ABP, and aniline were identified in exposed workers.
    • 4-aminobiphenyl (4ABP) and aniline were the major adducts found.
    • Bz workers exhibited 10-17 fold higher adduct levels compared to dye workers.
    • Strong correlations (r >= 0.89) were observed between 4ABP, Bz, and AcBz adducts.

    Conclusions:

    • The presence of 4ABP and aniline adducts indicates exposure to Bz, azo dyes, or their metabolic release.
    • Occupational exposure to Bz and azo dyes leads to significant arylamine burden in workers.
    • 4ABP and Bz are potential co-carcinogens in exposed workers, necessitating further investigation into bladder cancer risk.
    • Genetic factors, such as NAT1 and NAT2 variants, may complicate the understanding of Bz-related bladder cancer risk.