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Updated: Aug 11, 2026
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Published on: December 21, 2019
Generation of three-dimensional hepatocyte/gelatin structures with rapid prototyping system
Xiaohong Wang1, Yongnian Yan, Yuqiong Pan
1Key Laboratory for Advanced Manufacturing by Materials Processing Technology and Center of Organism Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing, China. wangxiaochong@tsinghua.edu.cn
Abstract:
Using rapid prototyping technology, three-dimensional (3D) structures composed of hepatocytes and gelatin hydrogel have been formed. This technique employs a highly accurate 3D micropositioning system with a pressure-controlled syringe to deposit cell/biomaterial structures with a lateral resolution of 10 microm. The pressure-activated micro-syringe is equipped with a fine-bore exit needle for which a wide variety of 3D patterns with different arrays of channels (through-holes) were created. More than 30 layers of a hepatocyte/gelatin mixture were laminated into a high spacial structure using this method. The laminated hepatocytes remained viable and performed biological functions in the construct for more than 2 months. The rapid prototyping technology offers potential for eventual high-throughout production of artificial human tissues or organs.
