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Cyclooxygenase 2 expression in otitis media with effusion.
Masashi Kakiuchi1, Hidetsugu Tsujigiwa, Yorihisa Orita
1Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
American Journal of Otolaryngology
|February 28, 2006
Summary
Cyclooxygenase 2 (COX-2) expression increases in the middle ear following endotoxin exposure, suggesting its involvement in otitis media with effusion (OME) development.
Area of Science:
- Otorhinolaryngology
- Inflammation Research
- Molecular Biology
Background:
- Otitis media with effusion (OME) is a common pediatric condition.
- The underlying mechanisms of OME pathogenesis require further elucidation.
Purpose of the Study:
- To investigate the role of cyclooxygenase 2 (COX-2) in the development of otitis media with effusion (OME).
Main Methods:
- An experimental model of endotoxin-induced OME was established in BALB/c mice.
- Middle ear COX-2 expression was analyzed using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH).
Main Results:
- COX-2 expression in the middle ear demonstrated a dose-dependent increase following endotoxin injection.
- In situ hybridization revealed distinct COX-2 expression patterns in the middle ear epithelium of mice with OME.
- COX-2 was not detected in the middle ears of healthy control mice.
Conclusions:
- COX-2 expression is upregulated in experimental OME.
- COX-2 may play a significant role in the pathological processes contributing to OME development.