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Updated: Aug 11, 2026

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
Published on: March 19, 2019
X-ray diffraction analysis and molecular-replacement solution of the cyan fluorescent protein dsFP483
Meitian Wang1, Hetal N Patel, Rebekka M Wachter
1Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.
Abstract:
A novel cyan fluorescent protein, dsFP483 from the coral Discosoma striata, has been crystallized. Diffraction data were collected to 2.1 A and processed in space group C2. Molecular-replacement methods were applied using the closely related red fluorescent protein DsRed as a search model. The asymmetric unit appears to contain six protein molecules (1.5 tetramers), five of which (83%) could be located by the molecular-replacement searches.
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