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Cloning, purification, crystallization and preliminary X-ray diffraction analysis of nitrile hydratase from the

Shinji Hourai1, Takeshi Ishii, Misao Miki

  • 1Sumitomo Chemical Co. Ltd Environmental Health Science Laboratory, 3-1-98 Kasugade-naka, Konohanaku, Osaka 554-8558, Japan. shinji-horai@ds-pharma.co.jp

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|March 3, 2006
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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...