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Alkaline phosphatase fusion proteins as tags for identifying targets for placental ligands

Heiner Müller1, Michael J Soares

  • 1Department of Obstetrics and Gynecology, University of Rostock, Germany.

Methods in Molecular Medicine
|March 4, 2006
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...