Silencing of filamin A gene expression inhibits Ca2+ -sensing receptor signaling
Chunfa Huang1, Zhenzhen Wu, Kristine M Hujer
1Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, 10701 East Blvd., 151W, Cleveland, OH 44106, USA. chunfa.huang@case.edu
Abstract:
Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+ -sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.
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