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Related Experiment Videos

Human papillomavirus testing with a liquid-based system: feasibility and comparison with reference diagnoses.

Christine Bergeron1, Frederic Cas, Francis Fagnani

  • 1Pasteur-Cerba Laboratory, Cergy Pontoise, France. bergeron@pasteur-cerba.com

Acta Cytologica
|March 7, 2006
PubMed
Summary

Cervical specimens in CYTO-screen liquid are suitable for oncogenic human papillomavirus (HPV) DNA detection using the Hybrid Capture II (HCII) technique. This method shows high sensitivity for detecting cervical intraepithelial neoplasia (CIN), especially in ASC-US cases.

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Area of Science:

  • Gynecologic Oncology
  • Molecular Diagnostics
  • Virology

Background:

  • Cervical cancer screening relies on detecting oncogenic human papillomavirus (HPV).
  • Accurate HPV DNA detection is crucial for timely diagnosis and management of cervical lesions.
  • Standardization of sample collection and processing methods is essential for reliable HPV testing.

Purpose of the Study:

  • To validate the use of cervical specimens collected in CYTO-screen fixative liquid for oncogenic HPV DNA detection.
  • To assess the performance of the Hybrid Capture II (HCII) technique with these specimens.
  • To compare HPV testing results with cytologic and histologic findings.

Main Methods:

  • A technical feasibility study evaluated three sample preparation methods for HCII: proteinase K digestion, PBS washing, and a commercial conversion kit.

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  • Cell stability was tested up to 90 days post-collection.
  • HPV testing results were compared to cytologic and histologic diagnoses in a retrospective analysis of 995 routine tests.

    • Main Results:

    • The HCII technique with direct proteinase K digestion was as effective as the commercial kit.
    • HPV testing demonstrated high sensitivity for detecting cervical intraepithelial neoplasia (CIN) 2 or worse (98-100% in LSIL and HSIL cases).
    • In cases of atypical squamous cells of undetermined significance (ASC-US), HPV testing showed 88% sensitivity for CIN 2 or worse, with a 99% negative predictive value.

    Conclusions:

    • Cervical specimens collected in CYTO-screen fixative liquid are suitable for oncogenic HPV DNA detection using the HCII technique.
    • The HCII method is a sensitive tool for detecting high-grade cervical lesions, particularly when used in conjunction with ASC-US cytologic findings.
    • This approach offers a feasible option for HPV testing within routine cervical screening programs.