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High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
Related RNA polymerase-binding regions in human RAP30/74 and Escherichia coli sigma 70
1Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Abstract:
RAP30/74 is a heteromeric general transcription initiation factor that binds to mammalian RNA polymerase II. The RAP30 subunit contains a region that is similar in amino acid sequence to the RNA polymerase-binding domain of the Escherichia coli transcription initiation factor sigma 70 (sigma 70). Mammalian RNA polymerase II specifically protected a serine residue in the sigma 70-related region of RAP30 from phosphorylation in vitro. In addition, human RAP30/74 bound to Escherichia coli RNA polymerase and was displaced by sigma 70. These results suggest that RAP30 and sigma 70 have functionally related RNA polymerase-binding regions.
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