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Updated: Aug 10, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
[Consumption of intracellular hydrogen peroxide in epithelial human amnion cells]
Abstract:
The method for determination of intracellular oxidants content has been proposed. The method is based on the analysis of changes of 2,7-dichlorodihydrofluorescein oxidation. The theoretical model of hydrogen peroxide diffusion and its consumption in the cell and quantitative determination of hydrogen peroxide gradient through the cellular membrane has been built taking into account the experimental data. The coefficient for H2O2 permeability through the plasma membrane of human amnion cells is (2.1 +/- 0.1) x 10(-4) cm x c(-1).
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