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Site-directed mutagenesis of La protease. A catalytically active serine residue
Amerik AYu1, V K Antonov, A E Gorbalenya
1Shemyakin Institute of Biorganic Chemistry, USSR Academy of Sciences, Moscow.
FEBS Letters
|August 5, 1991
Abstract:
Comparative sequence analysis of Escherichia coli ATP-dependent La protease led to the suggestion that Ser679 is the catalytically active enzyme residue. Site-directed mutagenesis Ser679----Ala, investigation of the cells containing the mutant plasmid, and study of the partially purified mutant protein produced results in favour of this suggestion.