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Affinity of integrin alpha 1 beta 1 from liver sinusoidal membranes for type IV collagen
S C Stamatoglou1, S Bawumia, S Johansson
1National Institute for Medical Research, London, UK.
FEBS Letters
|August 19, 1991
Abstract:
Hepatic sinusoidal membranes isolated from adult rats were extracted with detergent and fractionated on a wheat germ agglutinin affinity column. Bound glycoproteins were eluted with N-acetyl glucosamine and chromatographed on a type IV collagen affinity column. Recovery of the bound fraction by EDTA and analysis by SDS-PAGE revealed two glycoproteins with apparent molecular weights of 180,000 and 117,000. These were identified immunologically by Western blotting as the alpha and beta subunits of integrin alpha 1 beta 1.