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Updated: Aug 10, 2026
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Published on: February 22, 2017
Analysis of human immunodeficiency virus type 1 integration by using a specific, sensitive and quantitative assay
Norio Yamamoto1, Chika Tanaka, YuFeng Wu
1Department of Molecular Virology, 1-5-45 Yushima, 113-8519, Tokyo, Japan. norio.mmb@tmd.ac.jp
Abstract:
A novel real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type-1 (HIV-1) DNA with high specificity and sensitivity. This assay reproducibly allowed the detection of three copies of integrated HIV DNA in a background of 100,000 cell equivalents of human chromosomal DNA. The non-specific amplification of unintegrated HIV-1 DNA was significantly inhibited in this assay and the specificity of this assay was much higher than the previously reported method. This assay showed that kinetics in viral DNA synthesis was cell-type dependent and that the kinetics of HIV-1 DNA integration was very rapid in Jurkat T cell line. This method may provide new insights into the integration processes and be useful in evaluating future integrase inhibitors.
