Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evanescent-imaging-ellipsometry-based microarray reader.

Srivatsa Venkatasubbarao1, Neil Beaudry, Yanming Zhao

  • 1Intelligent Optical Systems, 2520 West 237 Street, Torrance, California 90505, USA. srao@intopsys.com

Journal of Biomedical Optics
|March 11, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ROS-responsive multifunctional hyaluronic acid/gelatin based adhesive hydrogel encapsulating baicalin and cerium dioxide nanozymes accelerates infected diabetic wounds healing.

International journal of biological macromolecules·2026
Same author

Left atrial and ventricular functional parameters by cardiovascular magnetic resonance for phenotyping hypertrophic cardiomyopathy: diagnostic and prognostic value.

Quantitative imaging in medicine and surgery·2026
Same author

Enhancing the Chemical Stability of Layered Oxides via P─O Bonds Coupling Lattice Oxygen Reactions.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Identifying Venous Insufficiency in Head and Neck Reconstruction Flaps Using Machine Learning and Deep Learning Methods.

Head & neck·2026
Same author

Nanoconfined Water Manipulated Selective Proton Storage in Layered Tungsten Oxides for Versatile Supercapacitor Diodes.

ACS nano·2026
Same author

Coordination-Tuned Layered Vanadium Oxide via Electroactive Organic Pre-Intercalation Enables High-Capacity, Long-Cycling Nonaqueous Calcium-Ion Batteries.

ACS applied materials & interfaces·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
Same journal

Time-of-flight fluorescence depth mapping using a spatiotemporal deep learning model.

Journal of biomedical optics·2026
Same journal

Erratum: Color-filter-array-based multispectral photoplethysmography optical sensor and its motion artifact correction algorithm (Erratum).

Journal of biomedical optics·2026
Same journal

Real-time fidelity assessment of fluorescence molecular imaging without reference images.

Journal of biomedical optics·2026
See all related articles

A new label-less ellipsometric imaging microarray reader detects biomolecule binding. This sensitive technique measures minute thickness and refractive index changes, offering an alternative to fluorescence-based methods.

Area of Science:

  • Biophotonics
  • Biosensing Technology
  • Surface Science

Background:

  • Microarray technology is crucial for high-throughput biological analysis.
  • Current microarray readers often rely on fluorescence labeling, which can be complex and costly.
  • There is a need for label-less, sensitive detection methods in genomics, proteomics, and diagnostics.

Purpose of the Study:

  • To develop and validate a novel label-less ellipsometric imaging microarray reader.
  • To demonstrate the reader's capability in detecting biomolecular binding events.
  • To optimize the system for high sensitivity and explore its potential applications.

Main Methods:

  • Utilized polarized light and total internal reflection to illuminate the microarray surface.
  • Measured changes in the polarization of reflected light caused by biomolecular binding.

Related Experiment Videos

  • Developed a prototype reader and calibrated its performance using complementary DNA (cDNA) microarrays.

    • Main Results:

    • The reader successfully detected binding events on cDNA microarrays.
    • Achieved high sensitivity, measuring refractive index changes as small as 0.0024.
    • Demonstrated the capability to measure thickness changes as low as 0.28 nm.
    • Identified optimal conditions for angle of incidence and substrate refractive index for enhanced sensitivity.

    Conclusions:

    • The developed ellipsometric imaging microarray reader is a sensitive, label-less detection platform.
    • This technique provides a viable alternative to fluorescence-based readers for various applications.
    • Potential applications include genomics, proteomics, diagnostics, and advanced sensing.