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Updated: Aug 10, 2026

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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
Published on: February 5, 2021
Assembling and cloning genes for fusion proteins using reverse transcription one-step overlap extension PCR method
Zsolt B Nagy1, Zoltán Varga-Orvos, Barnabás Szakál
1Laboratory of Functional Genomics, Biological Research Center, Hungarian Academy of Sciences, Szeged H-6701, Hungary.
Analytical Biochemistry
|March 11, 2006
Summary
No abstract available in PubMed .
Related Concept Videos
RACE - Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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