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Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
Published on: December 24, 2015
Aging of mesenchymal stem cell in vitro
Mandana Mohyeddin Bonab1, Kamran Alimoghaddam, Fatemeh Talebian
1Hematology-Oncology & BMT Research Center, Shariati Hospital, Tehran University of Medical Sciences, Iran. mohyeddin@sina.tums.ac.ir
BMC Cell Biology
|March 15, 2006
Summary
Mesenchymal stem cells (MSC) lose their characteristics and age during long-term in vitro culture. Early use in cell and gene therapy is recommended due to undetectable senescence onset and loss of stemness.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Cellular senescence
Background:
- Mesenchymal stem cells (MSC) are crucial for therapies but require in vitro expansion.
- Low frequency of MSC in bone marrow (BM) necessitates extensive cell culture.
- The impact of prolonged culture on MSC senescence is a critical clinical consideration.
Purpose of the Study:
- To evaluate the effects of long-term in vitro culture on mesenchymal stem cell senescence.
- To assess changes in MSC characteristics, including proliferation, telomere length, and differentiation potential over time.
Main Methods:
- Long-term in vitro culture of mesenchymal stem cells.
- Monitoring of population doublings (PD) and telomere length.
- Assessment of differentiation potential and morphological changes.
Main Results:
- Cultures were maintained for a mean of 118 days (9 passages).
- Significant decrease in PD (7.7 to 1.2) and telomere length (9.19 to 8.7 kbp) observed.
- Differentiation potential declined from the 6th passage; morphological changes indicated cellular aging.
Conclusions:
- Mesenchymal stem cells (MSC) undergo senescence early and subtly during in vitro culture.
- Stem cell characteristics are diminished concurrently with senescence.
- Recommend early consideration of MSC for cell and gene therapy applications.
