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Multiplexing RT-PCR for the detection of multiple miRNA species in small samples

Kaiqin Lao1, Nan Lan Xu, Vivian Yeung

  • 1Applied Biosystems, 850 Lincoln Centre Dr., Foster City, CA 94404, USA. laokq@appliedbiosystems.com

Biochemical and Biophysical Research Communications
|March 15, 2006
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...