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DNA fingerprinting in cattle using oligonucleotide probes.
J Buitkamp1, H Zischler, J T Epplen
1Institut für Tierzucht and Vererbungsforschung, Abteilung Haustiergenetik der Tierärztlichen Hochschule Hannover, Germany.
Animal Genetics
|January 1, 1991
Summary
DNA fingerprinting using simple tandem repeat probes provides precise individual identification in cattle. This method is also valuable for accurate cattle paternity testing, enhancing genetic management.
Area of Science:
- Molecular Biology
- Genetics
- Animal Science
Background:
- Oligonucleotide probes targeting simple tandem repeat (STR) sequences are established for generating individual-specific DNA fingerprints across various species.
- STR-based DNA fingerprinting has demonstrated efficacy in human and animal identification.
Purpose of the Study:
- To evaluate the utility of specific synthetic oligonucleotide probes for DNA fingerprinting in cattle.
- To assess the discriminatory power of these probes for individual identification and paternity testing in German cattle breeds.
Main Methods:
- Hybridization of 11 synthetic oligonucleotide probes to bovine genomic DNA.
- Digestion of DNA using restriction endonucleases HinfI, AluI, and HaeIII.
- Analysis of DNA fingerprint patterns and calculation of genetic parameters across three German cattle breeds.
Main Results:
- Two selected probes generated distinct DNA fingerprint patterns in the analyzed German cattle breeds.
- The number of polymorphic bands observed per individual ranged from 11 to 23.
- The probability of two unrelated individuals sharing an identical fingerprint was very low, ranging from 1.5 x 10(-7) to 2.4 x 10(-7).
Conclusions:
- The developed DNA fingerprinting procedure using STR probes enables precise individual identification in cattle.
- This technique serves as a valuable supplementary tool for reliable paternity testing in cattle populations.
- The high discriminatory power supports its application in genetic management and breeding programs.