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Updated: Aug 10, 2026

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
Published on: May 16, 2022
[Substance P regulates function of osteoclasts via neurokinin-1 receptor]
Ying-Ming Sun1, Song-Jiao Luo, Yu-Hui Zhao
1Dept. of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the effects of substance P on cultured rat osteoclasts.
Methods:
Neurokinin-1 (NK1) receptor expression in osteoclasts was examined by immunohitochemical method, and changes of bone resorption activity caused by substance P and NK1 receptor antagonists were detected by pit formation assay.
Results:
Immunoreactivity for NK1 receptor was distributed in the cytoplasm of osteoclasts. The average of pit formation areas significantly increased with addition of substance P (10(-7)-10(-4) mol/L) (P < 0.05), but the number of pitformations did not change (P > 0.05). NK1 receptor antagonists inhibited the enhancement of the bone resorption by substance P addition.
Conclusion:
The findings suggested that substance P may stimulate osteoclasts and result in bone resorption by the mediation of NK1 receptor.
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