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Published on: July 8, 2021
[Transcriptional regulation of dentin sialophosphoprotein by c-Jun/c-Fos]
Wen-Xi He1, Zhong-Ying Niu, Shou-Liang Zhao
1Dept. of Conservative Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Objective:
To investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein (DSPP) gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23.
Methods:
Endogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene.
Results:
c-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter.
Conclusion:
These findings suggest c-Jun and c-Fos down-regulated the transcription of DSPP gene as a transcriptional factor in odontoblast.
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