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Updated: Aug 10, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
[Analysis and application of excitation fluorescence intensity of blood]
Zhi-Min Zhao1, Lin-Feng Guo, Xiao-Lei Yu
1College of Science, Nanjing Uiversity of Aeronautics and Astronautics, Nanjing 210016, China.
Abstract:
The analysis technique of fluorescence is adopted to study the intensity of excitation fluorescence of blood in the present paper. The theoretical analysis and differences of normal and abnormal blood (hyperglycemia, hyperlipemia) are presented. The theoretical analysis was proved by experiment results. It was discovered that blood sugar consistency has an effect on blood fluorescence. In other words, with the same excitation wavelength and with blood sugar consistency increasing, the fluorescence intensity increases gradually. It is obvious that blood sugar is also a kind of fluorescein, and its consistency has an effect on fluorescence intensity, which is identical with the theoretical analysis, indicating that the experiment is successful, and it is possible to distinguish blood sugar consistency by comparing the fluorescence intensity in blood. It was also discovered that the higher the cholesterin content, the more intense the fluorescence. When excitation wavelength is especially 435 nm, the phenomenon is very obvious. The study paves a new way for the blood quick check and diagnosis of diseases.

