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Updated: Aug 9, 2026

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
[Epidemic outbreak of acute gastroenteritis due to norovirus with possible hydric origin]
Diego Almagro-Nieves1, Pilar Guisosa-Campos, Sonia Garrido-Almagro
1Distrito Sanitario Metropolitano de Granada, España.
Introduction:
To describe the clinical, microbiological and epidemiological investigations performed after an outbreak of acute gastroenteritis in a wedding.
Method:
A descriptive, case-control study was performed. An epidemiological survey (45 cases-28 controls) and inspection were carried out.
Results:
Affected individuals had diarrhea and vomiting. Water and ice were contaminated. Norovirus was isolated in only one stool sample. Statistically significant differences in water consumption were found in bivariate analysis (p = 0.02; ORc = 10.6; 95% CI: 1.20-220.05) and multivariate analysis (p = 0.04; ORa = 8.93; 95% CI: 1.03-77.41).
Conclusions:
The clinical and epidemiological characteristics and laboratory results indicate that the most probable cause of the outbreak was norovirus.
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10:48EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
11:09EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
Published on: September 11, 2016
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