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Updated: Aug 9, 2026

Extracellular Multi-Unit Recording from the Olfactory Nerve of Teleosts
Published on: October 6, 2020
Activity of L-alpha-amino acids at the promiscuous goldfish odorant receptor 5.24
Bolette Christiansen1, Petrine Wellendorph, Hans Bräuner-Osborne
1Department of Medicinal Chemistry, Danish University of Pharmaceutical Sciences, 2 Universitetsparken, DK-2100 Copenhagen, Denmark.
Abstract:
The goldfish odorant receptor 5.24 is a member of family C of G protein-coupled receptors and is closely related to the human receptor GPRC6A. Receptor 5.24 has previously been shown to have binding affinity for L-alpha-amino acids, especially the basic amino acids arginine and lysine. Here we report the agonist activities of the 20 proteinogenic L-alpha-amino acids, and L-ornithine and L-citrulline, measured in an intracellular calcium release assay in mammalian tsA cells. The results show that receptor 5.24 is broadly activated by 19 of the tested L-alpha-amino acids and displays a preference for basic amino acids.
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