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Related Experiment Videos

Compartmentalized Ras/MAPK signaling.

Adam Mor1, Mark R Philips

  • 1Department of Medicine, New York University Medical Center, New York, NY 10016-6402, USA. mora01@med.nyu.edu

Annual Review of Immunology
|March 23, 2006
PubMed
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Ras/MAPK pathway signaling occurs not only at the plasma membrane but also on intracellular membranes like the Golgi apparatus. This compartmentalization influences T cell activation and differentiation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Signal Transduction

Background:

  • Ras/MAPK pathway signaling is crucial for T cell activation, proliferation, and differentiation.
  • Historically, this signaling was thought to occur exclusively at the plasma membrane.
  • The plasma membrane is now understood as a mosaic of microdomains, enabling signaling compartmentalization.

Purpose of the Study:

  • To investigate the role of intracellular membranes in Ras/MAPK pathway signaling.
  • To explore the distinct signaling kinetics and activation pathways on different cellular compartments.
  • To demonstrate Ras/MAPK pathway as an example of compartmentalized signaling.

Main Methods:

  • Utilized genetically encoded fluorescent probes to monitor Ras GTP/GDP exchange.

Related Experiment Videos

  • Investigated signaling events on various intracellular membranes, including the Golgi apparatus.
  • Analyzed Ras activation kinetics in fibroblasts on both plasma membrane and Golgi.

    • Main Results:

    • Ras/MAPK signaling was detected on intracellular membranes, notably the Golgi apparatus.
    • Ras activation on the plasma membrane and Golgi exhibits distinct temporal profiles in fibroblasts.
    • Activation of Golgi-associated Ras involves PLCgamma and RasGRP1, potentially requiring retrograde trafficking.

    Conclusions:

    • The plasma membrane is not the sole platform for Ras/MAPK signaling.
    • Intracellular membranes, such as the Golgi, serve as critical sites for Ras/MAPK signal transduction.
    • The Ras/MAPK pathway exemplifies compartmentalized signaling, with distinct mechanisms and kinetics across different cellular locations.