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Published on: July 16, 2019
[A microsatellite marker linked to the stripe rust resistance gene YrV23 in the wheat variety Vilmorin23]
Yue-Bing Wang1, Shi-Chang Xu, Zhong Xu
1National Key Lab for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China. smilewang@163.com
Abstract:
Vilmorin23 is an internationally used differential host variety for studies on the interactions between wheat stripe rust and wheat. It contains the stripe rust resistance gene YrV23 and is potentially an important source of stripe rust resistance worldwide. SSR analysis was performed on the wheat NIL Taichuang 29*6/YrV23 carrying the resistant gene YrV23 against stripe rust, Vilmorin 23 and its recurrent parent Taichung 29. Fifty pairs of SSR primers on wheat chromosome 2B were screened and a reproducible polymorphic DNA fragment amplified by Xwmc356 was found. Genetic linkage was tested on 150 segregating F2 plants. It showed that the microsatellite marker Xwmc356 was linked to the resistance gene YrV23 with a genetic distance of 9.4 cM.
