Related Experiment Video
Updated: Aug 9, 2026
Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization
Published on: October 24, 2025
A simplified method for preparing IVF granulosa cells for culture
Derek K Lobb1, Edward V Younglai
1Department of Obstetrics and Gynecology, McMaster University, Room No. 3N52 Health Science Centre, 1200 Main St. W., L8N 3Z5, Hamilton, Ontario, Canada. lobbd@mcmaster.ca
Purpose:
To validate an expedited method for the removal of erythrocytes when preparing IVF granulosa-luteal cells for culture.
Methods:
Granulosa cells were enriched for culturing from follicular aspirates by density gradient centrifugation and by hypo-osmotic lysis treatments.
Results:
Cells prepared by either method showed the same cell viability and produced progesterone in similar quantities.
Conclusions:
Using hypo-osmotic lysis to remove erythrocytes does not impair granulosa cell viability or steroidogenesis. It avoids multiple density gradient centrifugations and washings, and yields IVF granulosa cells ready for culture efficiently.
