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Updated: Aug 2, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
A new method for secondary standard measurements with the aid of liquid scintillation counting
1Physikalisch-Technische Bundesanstalt (PTB), Department 6.1, Bundesallee 100, D-38116 Braunschweig, Germany. Karsten.Kossert@ptb.de
Abstract:
A new secondary standard measuring procedure based on liquid scintillation counting has been developed at PTB. In this procedure, the efficiency curve, i.e. the counting efficiency of the nuclide to be investigated as a function of the tritium counting efficiency, is-in contrast to the CIEMAT/NIST method-not calculated but determined by experiment. For the measurements, gamma-emitting activity standards are used which were calibrated by other methods. The procedure was successfully used to calibrate solutions of the radionuclides 54Mn, 57Co, 65Zn, 85Sr and 125I with small uncertainties.
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