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In vitro antifungal efficacy of four irrigants as a final rinse

Melissa L Ruff1, Scott B McClanahan, Britta S Babel

  • 1Naval Postgraduate Dental School, Bethesda, Maryland, USA.

Journal of Endodontics
|March 24, 2006
PubMed

Insights

The study found that 6% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX) demonstrated superior antifungal efficacy against Candida albicans compared to 17% EDTA and BioPure MTAD in vitro. Both NaOCl and CHX were equally effective.

Area of Science:

  • Endodontics
  • Microbiology
  • Dental Materials Science

Background:

  • Candida albicans is a common opportunistic pathogen in endodontic infections.
  • Effective intracanal disinfection is crucial for successful root canal treatment outcomes.
  • Antimicrobial efficacy of final irrigants requires thorough investigation.

Purpose of the Study:

  • To compare the in vitro antifungal efficacy of four final irrigants against Candida albicans.
  • To evaluate 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX), 17% ethylene-diaminetetraacetic acid (EDTA), and BioPure MTAD.

Main Methods:

  • Forty-eight single-rooted teeth were prepared and inoculated with Candida albicans.
  • Teeth were treated with NaOCl, CHX, EDTA, or MTAD as final rinses.
  • Colony-forming units were counted on agar plates to assess antifungal activity.

Main Results:

  • 6% NaOCl and 2% CHX exhibited statistically significant superior antifungal activity compared to MTAD and EDTA (p < 0.05).
  • NaOCl and CHX showed comparable efficacy against Candida albicans.
  • EDTA and MTAD demonstrated lower antifungal effectiveness in this in vitro model.

Conclusions:

  • 6% NaOCl and 2% CHX are highly effective final irrigants against Candida albicans.
  • MTAD and 17% EDTA are less effective as antifungal agents in this context.
  • These findings support the use of NaOCl or CHX for enhanced disinfection in endodontic procedures.

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