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Updated: Aug 9, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
The Tre2 oncoprotein, implicated in Ewing's sarcoma, interacts with two components of the cytoskeleton
Christophe Dechamps1, Stephane Bach, Daniel Portetelle
1Animal and Microbial Biology Unit, Gembloux Agricultural University, Avenue Maréchal Juin 6, Gembloux, B-5030, Belgium.
Abstract:
The product of the Tre2 oncogene, structurally related to the Ypt/RabGTPase-activating proteins (Ypt/RabGAP), is involved in various human cancers, including Ewing's sarcoma. In order to identify proteins interacting with the GAP part of this protein, we performed yeast two-hybrid screening of two libraries. Two components of the cytoskeleton were thus identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments. The proteins found to interact with the GAP region are the light regulatory chain of myosin II (Myl2) and LOC91256, a protein containing ankyrin repeats.
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