Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyrotropin-receptor interaction and cyclic AMP-mediated effects in thyroid cells.

S Lissitzky, G Fayet, B Verrier

    Advances in Cyclic Nucleotide Research
    |January 1, 1975
    PubMed
    Summary

    Thyroid-stimulating hormone (TSH) in vitro reorganizes thyroid cells into functional follicles. This study investigated TSH binding and its effects on thyroid cell metabolism, revealing insights into TSH

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of the toxicity of combustion smokes at the air-liquid interface: a comparison between two lung cell models and two exposure methods.

    Archives of toxicology·2025
    Same author

    QSPR models to predict the physical hazards of mixtures: a state of art.

    SAR and QSAR in environmental research·2023
    Same author

    COVID-19: A putative trigger for neuralgic amyotrophy.

    Revue neurologique·2021
    Same author

    [HIV1 Env N-glycans and their interactions with the immune system].

    Virologie (Montrouge, France)·2021
    Same author

    [Vaccines against HIV/AIDS].

    Virologie (Montrouge, France)·2021
    Same author

    Sublingual vaccination and delivery systems.

    Journal of controlled release : official journal of the Controlled Release Society·2021

    Area of Science:

    • Endocrinology and Cell Biology
    • Thyroid Gland Physiology
    • Hormone Receptor Interactions

    Background:

    • Thyroid cells in culture serve as a model for studying thyroid gland function.
    • Thyroid-stimulating hormone (TSH) is crucial for thyroid cell organization and function.
    • TSH influences iodine metabolism and receptor binding in thyroid cells.

    Purpose of the Study:

    • To investigate the mechanism of TSH action on thyroid cells in vitro.
    • To characterize TSH binding kinetics at both cellular and subcellular levels.
    • To correlate TSH binding with cellular responses like adenylate cyclase stimulation and iodide transport.

    Main Methods:

    • Utilized 125-I-labeled TSH of high purity and specific radioactivity.
    • Studied TSH interaction with intact thyroid cells and isolated plasma membranes.
    • Analyzed TSH binding kinetics (association, dissociation) and receptor characteristics.

    Main Results:

    • Identified a single type of high-affinity, low-capacity TSH binding site.
    • Observed biphasic dissociation kinetics of bound TSH (half-lives of 3 and 30 min).
    • Found a strong correlation between TSH binding constants and cellular responses (adenylate cyclase, iodide transport).

    Conclusions:

    • The in vitro thyroid cell culture system accurately reflects physiological TSH regulation.
    • TSH binding is mediated by a specific receptor and influences key metabolic pathways.
    • Cyclic AMP, new RNA, and protein synthesis are involved in TSH's morphogenetic and metabolic effects.

    Related Experiment Videos