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Updated: Jun 9, 2026

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
[A micro-culture method for continuous observation of free-living amoebae]
Hong-you Chen1, Qing-wu Jiang, Qin-xue Li
1Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.
Objective:
To establish a method for co-culture of amoebae and endosymbionts, also for continuously observing the microphenotype of amoebae.
Methods:
24 wells culture plate with cover glass on the wells was used as containers. Amoebae and Candida albicans were co-cultured in microdrop of medium in the wells at 37 degrees C, and observed under x1000.
Results:
Continuous observation revealed trophozoites in various shapes like letters T, K, or Y, their movement and ingestion phenomenon were observed.
Conclusion:
The micro-culture method is useful in observing the amoebal morphology and its phagocytic process to Candida albicans.
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