Related Experiment Video
Updated: Aug 9, 2026
The Pocket-Creation Procedure of Endoscopic Submucosal Dissection for Large Rectal Laterally Spreading Tumors
Published on: February 13, 2026
Using p53-immunostained large specimens to determine the distal intramural spread margin of rectal cancer
Zhi-Zhong Pan1, De-Sen Wan, Chang-Qing Zhang
1Department of Abdominal Surgery, Cancer Center, Sun Yat-Sen University, 651 Dongfeng Road, Guangzhou 510060, Guangdong Province, China. panzhizhong@medmail.com.cn
Aim:
To determine the distal intramural spread (DIS) margin of rectal cancer.
Methods:
Sixty-one p53-positive specimens of rectal cancer were used. After conventional hematoxylin and eosin (H&E) staining, the DIS margin of rectal cancer in large specimens was examined by immunohistochemistry. The patients were divided into A, B, C, and D groups. After a long-term follow-up, the survival curves of the four groups were estimated using the life table.
Results:
Fifty-one of the sixty-one cases (83.6%) had DIS. The extent of DIS ranged 0.11-3.5 cm; meanwhile the mean of DIS measured by H&E staining was 0.13 cm. The significant difference was found between the means (t=5.622, P<0.0001). Only 1 of 51 patients had DIS greater than 3 cm. The DIS was less than 1.0 cm in most rectal cancer patients. The long-term results indicated that the survival rate of the patients whose DIS was greater than 1.0 cm was lower than that of the patients whose DIS was less than 0.5 cm.
Conclusion:
Rectal cancer patients with DIS greater than 1.0 cm have poor prognosis.
