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Decay of mammalian hexokinase: characterization of the specific proteolytic activity
1Division of Pediatric Hematology and Oncology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Biochimica Et Biophysica Acta
|October 11, 1991
Abstract:
Hexokinase-degrading activity (HKDA) is associated with hexokinase throughout purification. It is not ubiquitin-dependent. With glucose present, HKDA requires MgATP. It is triggered by removal (or ATP-dependent consumption) of glucose, and masked when glucose is restored. PCMB, TLCK and TPCK suppress hexokinase and activate HKDA. Glucose removal and active-site blockage appear to induce HKDA.