Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ovine bronchial-derived relaxing factor: changes with development and hyperoxic ventilation.

Satyan Lakshminrusimha1, Frederick C Morin, Robin H Steinhorn

  • 1Division of Neonatology, Department of Pediatrics, Center for Developmental Biology of the Lung, State University of New York at Buffalo, Women and Children's Hospital of Buffalo, 219 Bryant St., Buffalo, New York 14222, USA. slakshmi@buffalo.edu

Journal of Applied Physiology (Bethesda, Md. : 1985)
|April 1, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Former ONTPD chairs perspective on the 2-year fellowship proposal.

Journal of perinatology : official journal of the California Perinatal Association·2026
Same author

We're going bananas! The effectiveness and safety of banana puree as an intervention for improving oral feeding in a level IV NICU.

Journal of perinatology : official journal of the California Perinatal Association·2026
Same author

Improving LISA Practice: An Ongoing Observational Quality Improvement Initiative Following Initiation of Less-Invasive Surfactant Administration in a Level IV NICU.

Children (Basel, Switzerland)·2026
Same author

Revisiting time to blood culture positivity: can we decrease antibiotic exposure in the NICU?

Journal of perinatology : official journal of the California Perinatal Association·2026
Same author

Correction: Plasma apolipoprotein A-I is a causal protective factor in sepsis.

Scientific reports·2026
Same author

Can We Use Simple Radiographic Measurements to Predict Need for Intervention in Neonatal Pneumothorax?

Children (Basel, Switzerland)·2026

A bronchial-derived relaxing factor (BrDRF) reduces pulmonary artery contractility in newborn and adult sheep, but not fetuses. This effect is nitric oxide (NO)-mediated postnatally and may involve other factors after hyperoxic ventilation.

Area of Science:

  • Pulmonary vascular physiology
  • Neonatal respiratory adaptation
  • Vascular smooth muscle pharmacology

Background:

  • Bronchial-derived relaxing factor (BrDRF) is suggested to decrease pulmonary artery (PA) contractility in newborn rats via nitric oxide (NO).
  • The developmental regulation and precise mechanisms of BrDRF action remain incompletely understood.

Purpose of the Study:

  • To investigate the effect of an adjacent bronchus on PA contractility to norepinephrine (NE) in fetal, neonatal, ventilated neonatal, and adult sheep.
  • To determine the role of NO in the BrDRF-mediated relaxation and its developmental changes.

Main Methods:

  • Sheep (fetal, neonatal, ventilated neonatal, adult) were studied using isolated PA rings with and without an attached bronchus (PA+Br).
  • Vascular contractility to NE was measured in the presence and absence of the NO synthase inhibitor N(omega)-nitro-l-arginine (l-NNA).

Related Experiment Videos

  • Contractions were quantified relative to KCl-induced contractions and absolute force.

    • Main Results:

    • An attached bronchus significantly diminished NE-induced PA contractions in neonatal, ventilated neonatal, and adult sheep, but not in fetal sheep.
    • Hyperoxic ventilation increased NE contractions in both PA and PA+Br preparations.
    • l-NNA enhanced NE contractions in PA+Br in postnatal lambs, abolishing the difference between PA and PA+Br in neonates, but not in hyperoxic ventilated neonates.

    Conclusions:

    • A developmentally regulated BrDRF exerts vascular effects postnatally but not in fetal life.
    • BrDRF-mediated relaxation is primarily NO-dependent in air-breathing neonatal lambs.
    • Hyperoxic ventilation may induce a non-NO-dependent relaxation pathway involving BrDRF, suggesting a role in postnatal pulmonary arterial reactivity adjustments.