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Evolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS200 distribution
J Stanley1, C S Jones, E J Threlfall
1NCTC Plasmid Genetics Unit, Central Public Health Laboratory, London, U.K.
FEMS Microbiology Letters
|July 15, 1991
Summary
The Salmonella-specific DNA insertion element IS200 is present in all Salmonella enteritidis strains. Its genomic location and copy number reveal distinct evolutionary lineages, aiding in Salmonella enteritidis epidemiology.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- Salmonella enteritidis is a significant foodborne pathogen.
- Understanding its genetic diversity is crucial for public health.
- The IS200 element is a mobile genetic element found in Salmonella.
Purpose of the Study:
- To investigate the presence, copy number, and genomic location of the IS200 element in Salmonella enteritidis phage type strains.
- To correlate IS200 profiles with population genetics and evolutionary origins.
- To assess the utility of IS200 profiling for subdividing Salmonella enteritidis strains.
Main Methods:
- Genomic DNA extraction from 27 Salmonella enteritidis phage type strains.
- Southern blot hybridization using a DNA probe internal to IS200.
- Analysis of restriction site variation around IS200 insertion sites.
Main Results:
- All examined Salmonella enteritidis strains contained IS200 elements.
- Three distinct hybridization patterns (IS200 profiles) were identified, corresponding to specific chromosomal loci.
- These profiles correlated with clonal lineages and evolutionary origins of the strains.
- Each lineage was associated with a major phage type of clinical importance in the UK.
Conclusions:
- The IS200 element is a conserved marker in Salmonella enteritidis.
- IS200 profiling provides a valuable tool for understanding Salmonella enteritidis population structure and evolution.
- The findings support the use of IS200 profiles for epidemiological subdivision and outbreak investigations.