A population genomics approach to unlock the genetic potential of lablab (Lablab purpureus (L.) Sweet), an underutilized tropical forage crop

Lablab is one of the conventionally grown multi-purpose crops that originated in Africa. It is an annual or short-lived perennial forage legume which has versatile uses (as a vegetable and dry seeds, as food or feed, or as green manure) but is yet to receive adequate research attention and hence remains underexploited. To develop new and highly productive lablab varieties, using genomics-assisted selection, the present study aimed to identify quantitative trait loci associated with agronomically important traits in lablab and to assess the stability of these traits across two different agro-ecologies in Ethiopia. Here, we resequenced one hundred and forty-two lablab accessions, utilised whole genome genotyping approaches, and conducted multi-locational phenotyping over two years.

The selected lablab accessions displayed significant agro-morphological variation in eight analysed traits, including plant height, total fresh weight, and total dry weight. Furthermore, the agronomic performance of the accessions was significantly different across locations and years, highlighting substantial genotype-by-environment interactions. The population genetic structure of the lablab accessions, based on ~ 500,000 informative single nucleotide polymorphisms (SNPs), revealed an independent domestication pattern for two-seeded and four-seeded lablab accessions. Finally, based on multi-environmental trial data, a genome-wide association study (GWAS) identified useful SNPs and k-mers for key traits, such as plant height and total dry weight.

The publicly available genomic tools and field evaluation data from this study will offer a valuable resource for plant breeders and researchers to inform a new cycle of lablab breeding. With the aid of these tools, the breeding cycle will be significantly reduced and livestock farmers will have access to improved lablab varieties in a shorter time-frame.