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Related Experiment Videos

TNT detection using multiplexed liquid array displacement immunoassays.

George P Anderson1, Solimar C Moreira, Paul T Charles

  • 1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA. ganderson@cbmse.nrl.navy.mil

Analytical Chemistry
|April 4, 2006
PubMed
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Detecting explosive contamination requires better methods. This study uses a Luminex100 flow cytometer for rapid, multiplexed immunoassay detection of trinitrotoluene (TNT) in environmental samples.

Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Trace explosive contamination in soil and groundwater is a significant environmental concern.
  • Effective detection and quantification methods are crucial for site characterization and remediation monitoring.
  • Immunosensors offer a promising approach for detecting environmental contaminants in solution-phase.

Purpose of the Study:

  • To develop and validate a multiplexed immunoassay for the rapid detection and quantification of trinitrotoluene (TNT) and other nitroaromatics.
  • To evaluate the performance of different TNT antibodies using the Luminex100 platform.
  • To assess the applicability of the developed assay in various environmental matrices.

Main Methods:

  • Utilized the Luminex100 flow cytometer for multiplexed immunoassay detection.

Related Experiment Videos

  • Employed a displacement immunoassay format to detect TNT.
  • Simultaneously evaluated four monoclonal TNT antibodies, two recombinant TNT antibodies, and a control antibody.
  • Tested assay performance in lake water, seawater, and soil extracts.

    • Main Results:

    • Successfully detected TNT at concentrations as low as 0.1 ppb.
    • Quantified TNT over a range of 1.0 ppb to 10 ppm.
    • Demonstrated assay effectiveness in diverse matrices including lake water, seawater, and soil extracts.
    • Identified optimal dilution factors for seawater and soil extracts to maintain assay performance.

    Conclusions:

    • The Luminex100-based multiplexed immunoassay provides a rapid and sensitive method for TNT detection.
    • The assay is adaptable to various environmental matrices, with appropriate sample preparation.
    • This technology holds potential for improved environmental monitoring and site characterization of explosive contamination.